Triazoles are the only compounds used as antibiotics in both medicine and agriculture. The presence of triazoles in the environment can contribute to the acquisition of azole resistance among isolates of . The objective of this study was to investigate the effect of exposure to triazoles on susceptibility to these compounds. Seventeen triazole-resistant and 21 triazole-sensitive isolates were examined. The isolates were transferred 20 times on the Sabouraud medium supplemented with posaconazole, itraconazole or voriconazole, followed by five times on the medium not supplemented. The minimum inhibitory concentrations of antimycotics were examined according to the EUCAST broth microdilution method after the 20th transfer and also the 25th transfer. In addition, the expression levels of genes , , , , A and B were determined. Cultivation of on media supplemented with posaconazole, itraconazole and voriconazole resulted in the acquisition of resistance to the tested triazoles of all examined isolates. After recultivation on Sabouraud without azoles, most of the isolates lost their acquired resistance. The long-term use of triazole compounds in agriculture may result in the occurrence of triazole resistant isolates in the environment, not only by induction or selection of mutations in the A gene, but also by contribution to changes in the gene expression.
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| http://dx.doi.org/10.3390/microorganisms8020285 | DOI Listing |
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