AI Article Synopsis
- DDR1 is a receptor that responds to collagen and is linked to poor outcomes in tumors, but its exact role in cancer development is not well understood.
- DDR1 expression is confirmed in several human cancer cell lines, and its activation leads to significant phosphorylation of proteins like AKT and ERK.
- Inhibiting DDR1 reduces cancer cell behaviors like adhesion, invasion, and response to collagen, highlighting its crucial role in promoting metastasis in human carcinomas.
Article Abstract
DDR1 is a receptor tyrosine kinases for collagen and an adverse prognostic factor in primary and metastatic tumors.Despite this, DDR1 signaling and its functional consequences in tumor development remain unclear. RT-PCR and Western blot show that A375, colon carcinoma HT29 and liver carcinoma SK-HEP human cell lines express functional DDR1 that phosphorylates in response to collagen type I. Chemical inhibition of DDR1 phosphorylation or DDR1 mRNA silencing reduced AKT and ERK phosphorylation, expression of ICAM1 and VCAM1, Ki67 and secretion of MMP9. DDR1 silenced cells showed reduced adhesion to collagen type I, MMP-dependent invasion, and chemotactic and proliferative responses to collagen type I. Our work indicates an essential role for DDR1 signaling in key prometastatic features of collagen type I in human carcinoma cells.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153652 | PMC |
| http://dx.doi.org/10.1080/19336918.2020.1733892 | DOI Listing |
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