AI Article Synopsis
- The study examined the safety and effectiveness of aPDT using Toluidine Blue O (TBO) and LED light on oral cells and compared it to traditional antibiotics for treating periodontitis-causing bacteria.
- The aPDT significantly reduced harmful bacteria like P. gingivalis, F. nucleatum, and A. actinomycetemcomitans, with cytotoxic effects comparable to standard antiseptics but no harm to beneficial oral bacteria.
- Results showed aPDT's bactericidal effectiveness was on par with a common antibiotic combination (amoxicillin + metronidazole), suggesting it could be a safe alternative for treating periodontitis without damaging healthy tissue
Article Abstract
Background: To determine the safety and efficacy of antimicrobial photodynamic therapy (aPDT) combination of 0.33 mM Toluidine Blue O (TBO) with 60 mW/cm LED irradiation for 5 min that we had established, this study investigated the cytotoxic effect of aPDT combination on mammalian oral cells (gingival fibroblast and periodontal ligament cells) and compared the antimicrobial efficacy of antibiotics (the combination of amoxicillin (AMX) and metronidazole (MTZ)) against representative periodontitis pathogenic bacteria (Porphyromonas gingivalis, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans) versus our aPDT combination.
Result: aPDT combination did not show any detectable effect on the viability of Streptococcus sanguinis or Streptococcus mitis, the most common resident species in the oral flora. However, it significantly reduced CFU values of P. gingivalis, F. nucleatum, and A. actinomycetemcomitans. The cytotoxicity of the present aPDT combination to mammalian oral cells was comparable to that of standard antiseptics used in oral cavity. In antimicrobial efficacy test, the present aPDT combination showed equivalent bactericidal rate compared to the combination of AMX + MTZ, the most widely used antibiotics in the periodontitis treatment. The bactericidal ability of the AMX + MTZ combination was effective against all five bacteria tested regardless of the bacterial species, whereas the bactericidal ability of the aPDT combination was effective only against P. gingivalis, F. nucleatum, and A. actinomycetemcomitans, the representative periodontitis pathogenic bacterial species.
Conclusion: The present study demonstrated the safety and efficacy of the present aPDT combination in periodontitis treatment. TBO-mediated aPDT with LED irradiation has the potential to serve as a safe single or adjunctive antimicrobial procedure for nonsurgical periodontal treatment without damaging adjacent normal oral tissue or resident flora.
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| http://dx.doi.org/10.1016/j.pdpdt.2020.101688 | DOI Listing |
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