AI Article Synopsis
- The study investigates antibody responses to Chlamydia trachomatis in nonhuman primates, specifically looking at macaques infected through different routes (intravaginally and ocularly).
- Sera from 12 macaques were analyzed, revealing that they recognized a total of 172 C. trachomatis antigens, with variations in recognition based on the macaque type and infection method used.
- The findings suggest that the presence of antibodies to the outer membrane complex B antigen (OmcB) may serve as a biomarker for more invasive C. trachomatis infections, highlighting a link between infection severity and antibody response.
Article Abstract
Aims: Nonhuman primates have been used to investigate pathogenic mechanisms and evaluate immune responses following Chlamydia trachomatis inoculation. This study aimed to systemically profile antibody responses to C. trachomatis infection in nonhuman primates.
Materials And Methods: Sera were obtained from 4 pig-tailed and 8 long-tailed macaques which were intravaginally or ocularly infected with live C. trachomatis organisms, and analyzed by C. trachomatis proteome array of antigens.
Key Findings: The sera from 12 macaques recognized total 172 C. trachomatis antigens. While 84 antigens were recognized by pig-tailed macaques intravaginally infected with serovar D strain, 125 antigens were recognized by long-tailed macaques ocularly infected with serovar A, and 37 antigens were recognized by both. Ocular inoculation with virulent A2497 strain induced antibodies to more antigens. Among the antigens uniquely recognized by A2497 strain infected macaques, outer membrane complex B antigen (OmcB) induced robust antibody response. Although macaques infected by less virulent A/HAR-13 strain failed to develop antibodies to OmcB, reinfection by A2497 strain induced high levels of antibodies to OmcB.
Significance: Proteome array has revealed a correlation of chlamydial infection invasiveness with chlamydial antigen immunogenicity, and identified antibody responses to OmcB potentially as biomarkers for invasive infection with C. trachomatis.
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| http://dx.doi.org/10.1016/j.lfs.2020.117444 | DOI Listing |
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