Re-track: Software to analyze the retraction and protrusion velocities of neurites, filopodia and other structures.

Anal Biochem

Department of Physics, Worcester Polytechnic Institute, 100 Institute Road, Worcester, MA, 01609, USA. Electronic address:

Published: May 2020

We have developed new software, Re-track, that will quantify the rates of retraction and protrusion of structures emanating from the central core of a cell, such as neurites or filopodia. Re-Track, uses time-lapse images of cells in TIFF format and calculates the velocity of retraction or protrusion of a selected structure. The software uses a flexible moving boundary and has the ability to correct this boundary throughout analysis. Re-Track is fast, platform independent, and user friendly, and it can be used to follow biological events such as changes in neuronal connections, tip-growing cells such as moss, adaptive migration of cells, and similar behavior in non-biological systems.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9195366PMC
http://dx.doi.org/10.1016/j.ab.2020.113626DOI Listing

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