Effects of Bisphenol A on gene expression and DNA damage in adult viviparous fish .

Bisphenol A (BPA) is an emerging pollutant of global concern. Viviparous fish is endemic to the Central Mexican Plateau where BPA was detected; however, few studies examined the influence of this chemical on native viviparous fish. The effects of BPA (sublethal dose) were determined on DNA integrity and expression in gonads, and interactions of BPA with FOXL2 protein. Genotoxicity analysis revealed that % comets, at 14 and 28 days and comet tail length (at 14 days) were significantly higher in exposed compared to controls. In general, the % DNA tail was not markedly higher in BPA-treated fish; however, tail moment related to tail length exhibited significant increases in DNA damage. RT-qPCR assays showed overexpression after 14 and 28 days of exposure in females; while in males, was overexpressed after 28 days. analysis demonstrated that BPA interacted with seven residues located in FOXL2 homeodomain. In summary, sublethal BPA doses induced DNA damage and changes in expression in gonadal cells of , which may adversely affect reproduction in BPA-exposed wild populations. overexpression and BPA-FOXL2 interaction suggested alterations in processes involving . Viviparous fish may thus serve as potential non-conventional models for assessing pollutants effects.