Effects of Radon From Hot Springs on Lymphocyte Subsets in Peripheral Blood.

Objective: To analyze changes in immune functions by detecting lymphocyte subsets in the peripheral blood of residents in the vicinity of radon from hot springs.

Methods: Two groups were randomly selected; 61 residents in the vicinity of the hot springs were assigned to the radon group, and 51 residents with a similar lifestyle and habits but no contact with hot springs were assigned to the control group. The percentages of lymphocyte subsets (CD3, CD4CD8, CD4CD8, CD4/CD8, and TCR/CD3) in the 2 groups were evaluated on a FACS Aria flow cytometer. The absolute values of lymphocytes (LYMPH#) and percentages of lymphocytes (LYMPH%) were measured by an automatic blood analyzer.

Results: In the radon group, the numbers of CD3 ( = -0.140, > .05) and CD4CD8 ( = -0.964, > .05) T cells were higher, as compared with the controls, but this difference was not significant. In addition, the number of CD4CD8 ( = -2.141, < .05) T cells was significantly lower in the radon group. Furthermore, the average ratios of CD4/CD8 ( = -2.201, < .05) and TCR/CD3 ( = 2.047, < .05) cells were significantly higher in the radon group than in the controls. Compared with the control group, the LYMPH# ( = -0.485, > .05) and LYMPH% (Z = -0.835, > .05) showed no significant change.

Conclusion: Radon-rich hot springs could alter the proportions of lymphocyte subsets and possibly affect immunologic functions.