In this work, ionic liquids-modified magnetic carboxymethyl cellulose nanoparticles (IL-MCMC) were prepared and used as supports for enzyme immobilization. The specific activity of immobilized lipase PPL-IL-MCMC was 1.43 and 2.81 folds higher than that of free PPL and PPL-MCMC, respectively. Water contact angle analysis indicated that the introduction of ionic liquids increased the hydrophobicity of supports, which in tune induced the lid-opening of lipase, allowing its active sites to become more accessible. In addition, the affinity between lipase and substrate immobilized on the prepared supports was enhanced. The same method was also applied to analyze immobilize penicillin G acylase (PGA) to further investigate the general applicability of the method. The results showed that the immobilized PGA exhibited higher stability than many other reported PGAs. The developed composites may be utilized as excellent supports for enzyme immobilization in industrial application.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.carbpol.2020.115914 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Unveiling the multifaceted potential of amyloid fibrils: from pathogenic myths to biotechnological marvels.
Biophys Rev
December 2024
Amity Institute of Molecular Medicine and Stem Cell Research, Amity University Uttar Pradesh, 201313 Noida, India.
Amyloid fibrils, historically stigmatized due to their association with diseases like Alzheimer's and Parkinson's, are now recognized as a distinct class of functional proteins with extraordinary potential. These highly ordered, cross-β-sheet protein aggregates are found across all domains of life, playing crucial physiological roles. In bacteria, functional amyloids like curli fibers are essential for surface adhesion, biofilm formation, and viral DNA packaging.
View Article and Find Full Text PDFImmobilized Nucleoside 2'-Deoxyribosyltransferases from Extremophiles for Nucleoside Biocatalysis.
ACS Omega
January 2025
School of Biology, University of St Andrews, North Haugh, St Andrews KY16 9ST, U.K.
The synthesis of nucleosides is crucial for pharmaceutical and biotechnological applications, acting as drugs and as essential building blocks for numerous therapeutic agents. However, most enzymes employed in nucleoside biocatalysis are not recycled, possess limited stability, and have strict substrate selection for ribonucleosides or 2'deoxyribonucleosides. We employed 2'-deoxyribonucleoside transferase (NDT) enzymes from thermophilic and psychrophilic bacteria to demonstrate they can be immobilized to enhance specific activity, stability, and recyclability.
View Article and Find Full Text PDFRapid screening of acetylcholinesterase inhibitors in Qi-Fu-Yin using magnetic metal-organic frameworks immobilized with acetylcholinesterase.
Bioorg Chem
January 2025
Faculty of Chinese Medicine and State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macao 999078 China; Innovative Institute of Chinese Medicine and Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355 China. Electronic address:
Current immobilization approaches for ligand fishing often experience challenges such as limited protein loading capacity and difficulties in the recycling process. To overcome these challenges, we synthesized a magnetic metal-organic frameworks (MMOFs) composite, which can be rapidly separated and has a large specific surface area, and employed it to immobilize acetylcholinesterase (AChE). The synthesized MMOFs@AChE composite exhibited a high immobilization yield (129.
View Article and Find Full Text PDFDegradation mechanism of PGNa by the immobilized degrading enzymes from magnetic fermentation residue biochar.
Sci Total Environ
January 2025
Engineering Research Center of Resource Utilization of Carbon-containing Waste with Carbon Neutrality, Ministry of Education, East China University of Science and Technology, Shanghai 200237, China; State Key Laboratory of Coal Liquification, Gasification and Utilization with High Efficiency and Low Carbon Technology, East China University of Science and Technology, Shanghai 200237, China. Electronic address:
The development of a method to efficiently remove high concentrations of penicillin G sodium (PGNa) from the environment is important for human and animal health and safety. In this study, the degradative enzymes were immobilized by adsorption using biochar from penicillin fermentation waste residue, which could efficiently remove PGNa (900 mg/L) from an aqueous solution, with a removal rate of 99.84 % within 20 min.
View Article and Find Full Text PDFSynthesis of chiral alcohol (S)-CHBE by co-immobilization of double enzymes based on organic-inorganic hybrid nanoflower.
Int J Biol Macromol
January 2025
Key Laboratory of Geriatric Nutrition and Health, Ministry of Education, Beijing Technology and Business University (BTBU), Beijing 100048, China; School of Light Industry Science and Engineering, Beijing Technology and Business University (BTBU), Beijing 100048, China. Electronic address:
The chiral alcohols (S)-4-chloro-3-hydroxy-butyric acid ethyl ester ((S)-CHBE) is a critical intermediate in the synthesis of various active pharmaceutical ingredients. This study presents the first investigation of the efficient production of (S)-CHBE using organic-inorganic hybrid nanoflowers (GDH-CR@HNFs) for the co-immobilization of glucose dehydrogenase (BsGDH) and carbonyl reductase (BsCR). By optimizing immobilization conditions, we significantly enhanced the catalytic activity and immobilization efficiency of the hybrid nanoflowers.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!