AI Article Synopsis
- Nosocomial Central Nervous System (CNS) infections can arise from neurosurgery and are challenging to diagnose, often relying on microbial cultures.
- This study assessed the FilmArray® Blood Culture Identification (BCID) panel, focusing on its ability to quickly identify common causes of sepsis in these infections.
- Results showed the BCID panel achieved a sensitivity of 77.4% and specificity of 100%, with improved sensitivity at 83.9% by adjusting the cut-off, indicating its potential as a valuable diagnostic tool, though more research is needed for clinical use.
Article Abstract
Nosocomial Central Nervous System (CNS) infections are often serious complications of neurosurgical procedures. Their diagnosis is complex and frequently based on microbiological culture. The aim of this work was to evaluate the effectiveness of the FilmArray® Blood Culture Identification (BCID) panel, a multiplex PCR designed to identify the most common etiologic agents of sepsis involved with nosocomial CNS infections. A total of ninety samples were analyzed with the BCID panel. The sensitivity and specificity achieved were 77.4% and 100% respectively, when compared with the reference method (culture). Based on the analysis of the melting curves, another cut-off was established improving sensitivity to 83.9% whilst maintaining 98.3% specificity. The BCID panel seems to be a helpful tool for the prompt diagnosis of CNS nosocomial infections. The cut-off proposed here can increase sensitivity, but further studies are required to confirm its effectiveness and its applicability in clinical microbiology laboratories.
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| http://dx.doi.org/10.1016/j.mimet.2020.105865 | DOI Listing |
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