AI Article Synopsis
- Traditional methods for extracting genomic DNA (gDNA) are slow, often taking hours, and usually require specialized equipment like fume hoods.
- A new optimized method called bead bashing-based (BBB) extraction allows for rapid gDNA extraction in just 12 minutes without the need for a fume hood.
- In tests with human tissue samples, this method produced a sufficient amount of gDNA for further applications, making it ideal for fast-paced clinical settings.
Article Abstract
Conventional genomic DNA (gDNA) extraction methods can take hours to complete, may require fume hoods and represent the most time-consuming step in many gDNA-based molecular assays. We systematically optimized a bead bashing-based (BBB) approach for rapid gDNA extraction without the need for a fume hood. Human tissue specimens (n = 34) subjected to the 12-min BBB method yielded 0.40 ± 0.17 (mean ± SD) μg of gDNA per milligram of tissue, sufficient for many downstream applications, and 3- and 6-min extensions resulted in an additional 0.43 ± 0.23 μg and 0.48 ± 0.43 μg per milligram of tissue, respectively. The BBB method provides a simple and rapid method for gDNA extraction from mammalian tissue that is applicable to time-sensitive clinical applications.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7252492 | PMC |
| http://dx.doi.org/10.2144/btn-2019-0172 | DOI Listing |
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