Sterile alpha and toll/interleukin receptor (TIR) motif-containing protein 1 (SARM1) plays a pivotal role in triggering the neurodegenerative processes that underlie peripheral neuropathies, traumatic brain injury, and neurodegenerative diseases. Importantly, SARM1 knockdown or knockout prevents degeneration, thereby demonstrating that SARM1 is a promising therapeutic target. Recently, SARM1 was shown to promote neurodegeneration via its ability to hydrolyze NAD, forming nicotinamide and ADP ribose (ADPR). Herein, we describe the initial kinetic characterization of full-length SARM1, as well as the truncated constructs corresponding to the SAMTIR and TIR domains, highlighting the distinct challenges that have complicated efforts to characterize this enzyme. Moreover, we show that bacterially expressed full-length SARM1 (/ = 6000 ± 2000 M s) is at least as active as the TIR domain alone (/ = 1500 ± 300 M s). Finally, we show that the SARM1 hydrolyzes NAD via an ordered uni-bi reaction in which nicotinamide is released prior to ADPR.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7085114 | PMC |
| http://dx.doi.org/10.1021/acs.biochem.9b01078 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Characteristics of humoral responses to the first coronavirus disease booster vaccine and breakthrough infection in central China: a multicentre, prospective, longitudinal cohort study.
Front Immunol
January 2025
Department of Special Laboratory, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, and People's Hospital of Henan University, Zhengzhou, China.
Introduction: The long-term immunogenicity, adverse effects, influencing factors, and protection from booster vaccines remain unclear. Specifically, little is known regarding the humoral immunity and breakthrough infections associated with COVID-19 booster immunization. Therefore, we evaluated the immunogenicity, reactogenicity, influencing factors, and protective effects of the first coronavirus disease booster vaccine 23 months before and after implementation of dynamic zero epidemic control measures among healthcare staff.
View Article and Find Full Text PDFDecorating channel walls in ordered macroporous ZIF-8 with hydrophilic PEG to immobilize lipase for efficient chiral resolution.
Int J Biol Macromol
January 2025
Department of Chemistry and Chemical Engineering, Hunan Institute of Science and Technology, Yueyang 414006, Hunan, China.
The development of efficient immobilization support for the enhancement of enzyme activity and recyclability is a highly desirable objective. Single-crystalline ordered macro-microporous ZIF-8 (SOM-ZIF-8), has emerged as a highly effective matrix for enzyme immobilization, however, the inherent hydrophobic nature limits its further advancement. Herein, we have customized the immobilization of the Pseudomonas cepacia lipase (LP) in the modification-channels of SOM-ZIF-8 by functionalizing the inner surface-properties with polyethylene glycol (PEG) (LP@SOM-ZIF-8-PEG), and significant enhancement of the activity and (thermal, solvent and cyclic) stability can be realized.
View Article and Find Full Text PDFTuning multi-scale pore structures in carbonaceous films via direct ink writing and sacrificial templates for efficient indoor formaldehyde removal.
J Hazard Mater
January 2025
Key Laboratory of Coastal Urban Resilient Infrastructures (Ministry of Education), College of Civil and Transportation Engineering, Shenzhen University, Shenzhen 518060, China; Beijing Key Laboratory of Indoor Air Quality Evaluation and Control, Department of Building Science, Tsinghua University, Beijing 100084, China; State Key Laboratory of Intelligent Geotechnics and Tunnelling, Shenzhen University, Shenzhen 518060, China; State Key Laboratory of Subtropical Building and Urban Science, Shenzhen 518060, China; Key Laboratory of Eco Planning & Green Building (Tsinghua University), Ministry of Education, Beijing 100084, China. Electronic address:
The primary challenges impeding the extensive application of adsorption for indoor air purification have been low efficiency and effective capacity. To fill the research gap, we developed carbonaceous net-like adsorption films featuring multi-scale porous structures for efficient indoor formaldehyde removal. By optimizing the interfacial mass transfer and internal diffusion, we designed macro- to mesoscale meshes on the film surface and micro- to nano-scale pores within the materials, which were achieved by direct-ink-writing (DIW) printing and sacrificial template methods, respectively.
View Article and Find Full Text PDFWeak H-Bond Interface Environment for Stable Aqueous Zinc Batteries.
ACS Nano
January 2025
School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore 639798, Singapore.
Hydrogen evolution reaction and Zn dendrite growth, originating from high water activity and the adverse competition between the electrochemical kinetics and mass transfer, are the main constraints for the commercial applications of the aqueous zinc-based batteries. Herein, a weak H-bond interface with a suspension electrolyte is developed by adding TiO nanoparticles into the electrolytes. Owing to the strong polarity of Ti-O bonds in TiO, abundant hydroxyl functional groups are formed between the TiO active surface and aqueous environment, which can produce a weak H-bond interface by disrupting the initial H-bond networks between the water molecules, thereby accelerating the mass transfer of Zn and reducing the water activity.
View Article and Find Full Text PDFSelective synthesis of triacylglycerols by the ADS-17-supported Candida antarctica lipase B through esterification of oleic acid and glycerol.
J Sci Food Agric
January 2025
School of Food Science, Guangdong Pharmaceutical University, Zhongshan, China.
Background: Immobilized enzyme possessing both high activity and good selectivity is important in practice. In this study, Candida antarctica lipase B (CALB) was immobilized onto the macroporous resin ADS-17 for triacylglycerol (TAG) synthesis through esterification of oleic acid and glycerol. The reaction conditions were optimized by single-factor study and orthogonal test, and the reusability of the immobilized CALB (CALB@ADS-17) was evaluated.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!