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Genetic factors regulating Plasmodium falciparum gametocytogenesis identified by phenotypic screens.
Sci Rep
December 2024
Center for Global Health and Inter-Disciplinary Research, College of Public Health, University of South Florida, Tampa, FL, USA.
Successful transmission of Plasmodium falciparum from one person to another relies on the complete intraerythrocytic development of non-pathogenic sexual gametocytes infectious for anopheline mosquitoes. Understanding the genetic factors that regulate gametocyte development is vital for identifying transmission-blocking targets in the malaria parasite life cycle. Toward this end, we conducted a forward genetic study to characterize the development of gametocytes from sexual commitment to mature stage V.
View Article and Find Full Text PDFSingle-chain antibody gene therapy strategy based on high-throughput screening triggers sustained antiviral activity in the body.
J Virol
December 2024
College of Animal Science and Technology, Northwest A&F University College of Animal Science and Technology, Yangling, China.
The occurrence of viral diseases poses a huge threat and impact on human public health safety and the development of the animal and fishery industry. Here, a strain of single-chain antibody fragment, scFv-1, was isolated from the phage antibody display library construct by immunizing New Zealand white rabbits with rhabdovirus. analysis showed that the single-chain antibody could inhibit the infection of the virus in multiple pathways, including adsorption, fusion, and release.
View Article and Find Full Text PDFCRISPR/Cas9 has massively accelerated the generation of gene loss-of-function models in zebrafish. However, establishing tissue-specific mutant lines remains a laborious and time-consuming process. Although a few dozen tissue-specific Cas9 zebrafish lines have been developed, the lack of standardization of some key methods, including gRNA delivery, has limited the implementation of these approaches in the zebrafish community.
View Article and Find Full Text PDFUnderstanding the biosynthesis, metabolic regulation, and anti-phytopathogen activity of 3,7-dihydroxytropolone in spp.
mBio
October 2024
Department of Molecular Microbiology, John Innes Centre, Norwich, United Kingdom.
The genus is a prolific source of specialized metabolites with significant biological activities, including siderophores, antibiotics, and plant hormones. These molecules play pivotal roles in environmental interactions, influencing pathogenicity, inhibiting microorganisms, responding to nutrient limitation and abiotic challenges, and regulating plant growth. These properties mean that pseudomonads are suitable candidates as biological control agents against plant pathogens.
View Article and Find Full Text PDFGenome-wide CRISPRi screens for high-throughput fitness quantification and identification of determinants for dalbavancin susceptibility in .
mSystems
July 2024
Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Norway.
Article Synopsis
- Antibiotic resistance is a significant issue for treating staphylococcal infections, prompting the need for new strategies to enhance antibiotic effectiveness.
- The study developed a CRISPR interference library to pinpoint genes influencing antibiotic susceptibility, particularly targeting the antibiotic dalbavancin, and identified both known and previously unrecognized genes involved in antibiotic tolerance.
- Findings suggest that certain gene knockdowns, like KapB, can specifically increase sensitivity to dalbavancin, offering insights that could lead to novel antimicrobial treatments.
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