Wild cotton species are an important source of desirable genes for genetic improvement of cultivated cotton Linnaeus, 1763. For the success of such an improvement, chromosome pairings and recombinations in hybrids are fundamental. The wild African species Hutchinson & Lee, 1958 could be used as donor of the desirable trait of fiber fineness. Twelve BC1 plants obtained from the backcrossing of [( × Todaro, 1877) × ] (ADDF, 2n = 4x = 52) trispecies hybrid (HTL) by (cv. C2) (AADD, 2n = 4x = 52) were investigated for meiotic behaviour and plant fertility. Their chromosome associations varied as follows: (2.5 to 11.5) I + (17 to 22) II + (0.31 to 1.93) III + (0.09 to 1.93) IV + (0 to 0.07) V + (0 to 0.14) VI. Their pollen fertility ranged from 4.67 to 32.10 %. Only four BC1 plants produced a few seeds through self-pollination. The remaining BC1 were totally self-sterile and usually presented the highest number of univalents. All BC1 materials produced BC2 seeds (0.44 to 6.50 seeds per backcross) with the number of seeds negatively correlated with the number of univalents (R = 0.45, P < 0.05). Most BC1 plants gave significantly finer fiber compared to the cultivated . SSR markers showed a segregation of wild alleles among the backcross derivatives and Genomic hybridization (GISH) revealed presence of entire chromosomes of as well as recombinant chromosomes in the backcross derivatives. The significance and details of these results are presented and the prospects of successfully exploiting these plant materials are discussed.
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http://dx.doi.org/10.3897/CompCytogen.v14i1.47231 | DOI Listing |
Front Plant Sci
November 2024
Key Laboratory of Biohazard Monitoring, Green Prevention and Control for Artificial Grassland, Ministry of Agriculture and Rural Affairs, Institute of Grassland Research of Chinese Academy of Agricultural Sciences, Hohhot, China.
Front Plant Sci
November 2024
Plant Physiology Laboratory, Institute of Biology, Université de Neuchâtel, Neuchâtel, Switzerland.
Introduction: The Arabidopsis (Activity of BC1 complex/proton regulation 6) mutant is characterized by photosynthetic and conditional developmental phenotypes triggered by stressful red as well as high light. The Arabidopsis ABC1-like kinases belong to the atypical kinase family and contain conserved ATP-binding and hydrolysis motifs, but their physiological requirement has never been investigated.
Methods: By mutation to asparagine, we demonstrate that the highly conserved active site aspartate residue within ATP-binding motif VIIb is required for the physiological functions of ABC1K1.
Anim Genet
November 2024
Plant and Animal Genomics, Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB, Bellaterra, Spain.
The intramuscular oleic-to-stearic fatty acid ratio (C18:1n-9/C18:0) is an important indicator of the biosynthesis and desaturation of fatty acids in muscle. By using an RNA-Seq approach in muscle samples from 32 BC1_DU (25% Iberian and 75% Duroc) pigs with divergent values (high: H and low: L) of C18:1n-9/C18:0 fatty acids ratio, a total of 81 differentially expressed genes (DEGs) were identified. Functional analyses of DEGs indicate that mainly peroxisome proliferator-activated receptor signaling pathway (associated genes: PPARG, SCD, PLIN1, and FABP3) was overrepresented.
View Article and Find Full Text PDFJ Agric Food Chem
December 2024
State Key Laboratory of Green Pesticide, International Joint Research Center for Intelligent Biosensor Technology and Health, Central China Normal University, Wuhan 430079, PR China.
Cucumber downy mildew (CDM), caused by , is a destructive disease that affects greenhouse cucumbers and causes significant losses for growers. Amisulbrom, a triazole sulfonamide fungicide targeting the Qi site in the complex, has shown potential in effectively combating CDM. However, its detailed binding mode with the target is unclear.
View Article and Find Full Text PDFCarbohydr Polym
January 2025
College of Engineering, China Agricultural University, Beijing 100083, China. Electronic address:
Confocal Raman microscopy (CRM) is a promising in-situ visual technique that provides detailed insights into multiple lignocellulosic components and structures in plant cell walls at the micro-nano scale. In this study, we propose a novel CRM cosine similarity (CS) mapping strategy for the simultaneous in-situ visual profiling of lignin, cellulose, and hemicellulose in plant cell walls. The main stages of this strategy include: 1) a modified Otsu algorithm for extracting the regions of interest (ROI); 2) a modified subtraction method for cleaning the background signals in the ROI spectra; 3) a lignin signal subtraction method based on the pixel correction factor for eliminating the interference of strong lignin signals with weak cellulose and hemicellulose signals in the Raman full spectra of the cell walls; 4) second-order derivative spectral preprocessing for enhancing the discrimination between the characteristic peaks of cellulose and hemicellulose; 5) a CS mapping algorithm for simultaneous in-situ profiling of lignin, cellulose, and hemicellulose in plant cell walls.
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