Contraction fatigability during interleaved neuromuscular electrical stimulation of the ankle dorsiflexors does not depend on contraction amplitude.

Interleaved neuromuscular electrical stimulation (iNMES) involves alternating stimulus pulses between the tibialis anterior muscle and common peroneal nerve. The current investigation aimed to characterize the relationship between contraction amplitude, motor unit (MU) "overlap", and contraction fatigability during iNMES. It was hypothesized that as iNMES generates progressively larger contractions, more MUs would be recruited from both sites (i.e., more MU overlap), resulting in more fatigability for larger than smaller contractions. Fourteen participants completed 3 sessions. Fatigability was assessed as the decline in torque over 180 contractions (0.3 s "on", 0.7 s "off") when iNMES was delivered to produce initial contractions of ∼5%, 15%, or 30% of a maximal voluntary contraction. Although MU overlap increased significantly with contraction amplitude, the relative (percent) decline in torque was not different between the contraction amplitudes and torque declined on average by 23%. Contraction fatigability was not significantly correlated with either MU overlap or initial contraction amplitude. In conclusion, iNMES can produce fatigue-resistant contractions across a functionally-meaningful range of contraction amplitudes for rehabilitation. Interleaved neuromuscular electrical stimulation progressively recruits MUs as contraction amplitude increases. However, the relative amount of fatigability of recruited MUs was not different as contraction amplitude increased. This suggests iNMES can be used effectively to produce fatigue-resistant and functionally meaningful contractions.