Intracellular Behaviour of Three Strains within Three Amoeba Strains, Including C2c Maky.

is a facultative intracellular pathogen found in aquatic environments as planktonic cells within biofilms and as intracellular parasites of free-living amoebae such as . This pathogen bypasses the elimination mechanism to replicate within amoebae; however, not all amoeba species support the growth of . C2c Maky, a non-pathogenic amoeba, was previously demonstrated to possess the ability to eliminate the strain Paris. Here, we study the intracellular behaviour of three strains (Paris, Philadelphia, and Lens) within C2c Maky and compare this strain to and Z503, which are used as controls. We observe the intracellular growth of strain Lens within Z503 and at 22 °C and 37 °C. Strain Paris grows within at any temperature, while it only grows at 22 °C within Z503. Strain Philadelphia proliferates only within at 37 °C. Within C2c Maky, none of the three legionella strains exhibit intracellular growth. Additionally, the ability of C2c Maky to decrease the number of internalized is confirmed. These results support the idea that C2c Maky possesses unique behaviour in regard to strains.