AI Article Synopsis
- Advances in synthetic biology and metabolic engineering enable the creation of complex microbial synthesis systems, but individual cells often face limitations in machinery and resources.
- Coculture fermentation offers a solution by allowing different microbial populations to share production tasks, though traditional methods mainly focus on adjusting initial cell mixes.
- A new growth-regulation circuit based on quorum sensing significantly improved naringenin production, achieving a 60% increase in yield, and when combined with a communication module, led to an additional 60% boost in production efficiency.
Article Abstract
As synthetic biology and metabolic engineering tools improve, it is feasible to construct more complex microbial synthesis systems that may be limited by the machinery and resources available in an individual cell. Coculture fermentation is a promising strategy for overcoming these constraints by distributing objectives between subpopulations, but the primary method for controlling the composition of the coculture of production systems has been limited to control of the inoculum composition. We have developed a quorum sensing (QS)-based growth-regulation circuit that provides an additional parameter for regulating the composition of a coculture over the course of the fermentation. Implementation of this tool in a naringenin-producing coculture resulted in a 60% titer increase over a system that was optimized by varying inoculation ratios only. We additionally demonstrated that the growth control circuit can be implemented in combination with a communication module that couples transcription in one subpopulation to the cell-density of the other population for coordination of behavior, resulting in an additional 60% improvement in naringenin titer.
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| http://dx.doi.org/10.1021/acssynbio.9b00451 | DOI Listing |
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