AI Article Synopsis

  • The study focuses on the role of pancreatic β cells in insulin secretion and glucose regulation, emphasizing the need for direct measurement of β cell mass for better understanding of diabetes progression.
  • The research introduces a new multimodal probe that enables both fluorescence imaging and PET scans of pancreatic β cells, allowing for real-time monitoring and analysis without traditional pre-labeling methods.
  • This innovative approach facilitates faster and more efficient visualization of pancreatic islets and even monitors transplanted islets in live subjects, marking a significant advancement in diabetes research and treatment.

Article Abstract

Pancreatic β cells are responsible for insulin secretion and are important for glucose regulation in a healthy body and diabetic disease patient without prelabeling of islets. While the conventional biomarkers for diabetes have been glucose and insulin concentrations in the blood, the direct determination of the pancreatic β cell mass would provide critical information for the disease status and progression. By combining fluorination and diversity-oriented fluorescence library strategy, we have developed a multimodal pancreatic β cell probe for both fluorescence and for PET (positron emission tomography). By simple tail vein injection, stains pancreatic β cells specifically and allows intraoperative fluorescent imaging of pancreatic islets. -injected pancreatic tissue even facilitated an antibody-free islet analysis within 2 h, dramatically accelerating the day-long histological procedure without any fixing and dehydration step. Not only islets in the pancreas but also the low background of in the liver allowed us to monitor the intraportal transplanted islets, which is the first in vivo visualization of transplanted human islets without a prelabeling of the islets. Finally, we could replace the built-in fluorine atom in with radioactive 18F and successfully demonstrate in situ PET imaging for pancreatic islets.

Download full-text PDF

Source
http://dx.doi.org/10.1021/jacs.9b11173DOI Listing

Publication Analysis

Top Keywords

pancreatic cells
12
pancreatic
8
fluorescence pet
8
prelabeling islets
8
pancreatic cell
8
imaging pancreatic
8
pancreatic islets
8
islets
7
multimodal imaging
4
imaging probe
4

Similar Publications

The hypoxic microenvironment is an essential feature of solid tumors. Autophagy has been controversial in its role in immune regulation. This project aims to elucidate the impact of autophagy in pancreatic cancer (PC) under specific conditions (hypoxia) on CD8 T cells and the regulatory mechanisms behind it.

View Article and Find Full Text PDF

Diabetes is a medical condition, which belongs to the group of chronic diseases that affects how body processes glucose, the primary source of energy for cells. Glucose comes indirectly from the consumed food  and is carried by bloodstream to various cells in body. Insulin, a hormone synthesized by pancreas play a vital role in conversion of glucose to energy.

View Article and Find Full Text PDF

Are Probiotics Beneficial or Harmful for Pancreatic Cancer Outcomes?

Probiotics Antimicrob Proteins

December 2024

Division of Gastroenterology, Department of Internal Medicine and Liver Research Institute, Seoul National University Hospital, Seoul National University College of Medicine, 101, Daehak-Ro, Jongno-Gu, Seoul, Republic of Korea.

Pancreatic cancer is influenced by interactions between cancer cells and the tumor microenvironment (TME), including tumor-infiltrating lymphocytes (TILs). Specifically, CD8 + T cells impact prognosis by eliminating cancer cells. Recent studies have revealed that microbiomes are present in pancreatic tissues and may affect tumor growth and immune responses.

View Article and Find Full Text PDF

Pulmonary lysyl oxidase expression and its role in seeding Lewis lung carcinoma cells.

Clin Exp Metastasis

December 2024

Christopher S. Bond Life Sciences Center 540F, University of Missouri, 1201 E Rollins, Columbia, MO, 65211, USA.

Copper promotes tumor growth and metastasis through a variety of mechanisms, most notably as a cofactor within the lysyl oxidase (LOX) family of secreted cuproenzymes. Members of this family, which include LOX and LOX-like enzymes LOXL1-4, catalyze the copper-dependent crosslinking of collagens and elastin within the extracellular matrix (ECM). Elevated LOX expression is associated with higher incidence and worse prognosis in multiple cancers, including colorectal, breast, pancreatic, and head and neck.

View Article and Find Full Text PDF

Background: Pancreatic adenocarcinoma (PAAD) is a deadly cancer marked by extensive collagen deposition and limited response to immunotherapy. Discoidin domain receptor1 (DDR1), part of the transmembrane receptor tyrosine kinase family, is linked to inflammation regulation and immune cell infiltration. However, its role in controlling cytokines and chemokines in the microenvironment of PAAD is still unclear.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!

A PHP Error was encountered

Severity: Notice

Message: fwrite(): Write of 34 bytes failed with errno=28 No space left on device

Filename: drivers/Session_files_driver.php

Line Number: 272

Backtrace:

A PHP Error was encountered

Severity: Warning

Message: session_write_close(): Failed to write session data using user defined save handler. (session.save_path: /var/lib/php/sessions)

Filename: Unknown

Line Number: 0

Backtrace: