The present study investigated the anti-proliferative and chemosensitizing effects of var. against multi-drug resistant (MDR) cancer cells. The 80% methanol extract, chloroform (CHCI) fraction and butanol (BuOH) fraction of inhibited the growth of mitoxantrone (MX) resistant HL-60 (HL-60/MX2) cells. When HL-60/MX2 cells were treated with the CHCI and BuOH fractions, DNA ladder and sub-G1 hypodiploid cells were observed. Furthermore, the fractions reduced Bcl-2 mRNA levels, whereas Bax mRNA levels were increased. These results suggest that the inhibitory effect of on the growth of the HL-60/MX2 cells might arise from the induction of apoptosis. Treatment of HL-60/MX2 cells with the fractions markedly decreased the mRNA levels of the multi-drug resistance protein-1 and breast cancer resistance protein. The CHCI3 fraction and hexane fraction increased MX accumulation in HL-60/MX2 cells. These results imply that the CHCI fraction of plays a pivotal role as a chemosensitizer. We suggest that components of might have a therapeutic potential for the treatment of MDR leukemia.
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| http://dx.doi.org/10.5487/TR.2008.24.1.029 | DOI Listing |
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