Inhibitors of urease and ammonia monooxygenase can limit the rate of conversion of urea to ammonia and ammonia to nitrate, respectively, potentially improving N fertilizer use efficiency and reducing gaseous losses. Winter wheat grown on a sandy soil in the UK was treated with urea fertilizer with the urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT), the nitrification inhibitor dicyandiamide (DCD) or a combination of both. The effects on soil microbial community diversity, the abundance of genes involved in nitrification and crop yields and net N recovery were compared. The only significant effect on N-cycle genes was a transient reduction in bacterial ammonia monooxygenase abundance following DCD application. However, overall crop yields and net N recovery were significantly lower in the urea treatments compared with an equivalent application of ammonium nitrate fertilizer, and significantly less for urea with DCD than the other urea treatments.
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http://dx.doi.org/10.1007/s00374-019-01411-5 | DOI Listing |
Proc Natl Acad Sci U S A
January 2025
Departments of Molecular Biosciences and of Chemistry, Northwestern University, Evanston, IL 60208.
Methane- and ammonia-oxidizing bacteria play key roles in the global carbon and nitrogen cycles, respectively. These bacteria use homologous copper membrane monooxygenases to accomplish the defining chemical transformations of their metabolisms: the oxidations of methane to methanol by particulate methane monooxygenase (pMMO) and ammonia to hydroxylamine by ammonia monooxygenase (AMO), enzymes of prime interest for applications in mitigating climate change. However, investigations of these enzymes have been hindered by the need for disruptive detergent solubilization prior to structure determination, confounding studies of pMMO and precluding studies of AMO.
View Article and Find Full Text PDFWaste Manag
December 2024
State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China. Electronic address:
Biofiltration is an important method for composting off-gas treatment. Compost-based materials are widely used as the filling media for biofilter. To expand the application of compost from different composting materials in off-gas control for organic waste aerobic composting, the NH removal efficiency, NO generation, and microbial communities of ammonia monooxygenase (amoA functional gene was selected) and nitrite reductase (nirS functional gene was selected) were investigated using the animal manure compost (AMC) and sludge compost (SC) as filling materials.
View Article and Find Full Text PDFJ Appl Microbiol
December 2024
Department of Microbiology, University of Massachusetts, Amherst, MA, USA.
J Phys Chem B
December 2024
Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Stockholm SE-106 91, Sweden.
The oxidation of hydroxylamine was studied by quantum chemical modeling. Hydroxylamine is the product of ammonia oxidation in ammonia monooxygenase. That mechanism has been studied recently by quantum chemical modeling as here.
View Article and Find Full Text PDFEnviron Res
December 2024
College of Environmental & Resource Sciences, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou, 310058, China.
Granular size induces the operation performance variation of aerobic granular sludge reactor, but the profound reasons are unrevealed. This study investigated the influence of granular size distribution on the reactor operation under salt stress. The effective nitrogen removal was achieved at ≤4% salinity, but declined at 6% salinity.
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