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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7005800PMC
http://dx.doi.org/10.1038/s41598-020-59351-2DOI Listing

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Purifying membrane proteins has been the limiting step for studying their structure and function. The challenges of the process include the low expression levels in heterologous systems and the requirement for their biochemical stabilization in solution. The human voltage-gated proton channel (hH1) is a good example of that: the published protocols to express and purify hH1 produce low protein quantities at high costs, which is an issue for systematically characterizing its structure and function.

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