To clarify mechanisms affecting the level and distribution of 5-hydroxymethylcytosine (5hmC) during aging. We examined levels and genomic distribution of 5hmC along with the expression of ten-eleven translocation methylcytosine dioxygenases (TETs) in adipose stem cells in young and age-advanced individuals. 5hmC levels were higher in adipose stem cells of age-advanced than young individuals (p = 0.0003), but were not associated with age-related changes in expression of TETs. 5hmC levels correlated with population doubling time (r = 0.62; p = 0.01). We identified 58 differentially hydroxymethylated regions. Hypo-hydroxymethylated differentially hydroxymethylated regions were approximately twofold enriched in CCCTC-binding factor binding sites. Accumulation of 5hmC in aged cells can result from inefficient active demethylation due to altered TETs activity and reduced passive demethylation due to slower proliferation.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.2217/epi-2019-0131 | DOI Listing |
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!