Proton NMR and HR-LC/MS based phytochemical analysis of methanolic fraction of A. Rich. rhizomes.

Introduction: (Scrophulariaceae/Orobanchaceae) is a rarely occurring parasitic plant grows on roots of L. (Verbenaceae). As per Indian system of medicine, Ayurveda, it can be used in treatment of various diseases. So far, this plant has not been explored phyto-chemically in detail. Non-small cell lung cancer (NSCLC) is mostly occurring type of lung cancer, which so far can be treated by chemotherapy approach including cisplatin.

Aim: Present research work was aimed towards preparation of methanolic fraction of rhizomes; its phytochemical analysis by 1H-NMR and HR-LC/MS; evaluation of its anti-cancer property against NSCLC-A549 cell line.

Methods: For preparation of methanolic fraction (MF), rhizome powder was defatted; extracted with combination of water and alcohol (1:1); added with lead acetate and then sulphuric acid; fractionation of ethyl acetate fraction with methanol. After phytochemical analysis of MF by preliminary chemical testing, TLC, H-NMR and HR-LC/MS techniques, MF was screened for its anti - cancer property against NSCLC-A549 cell line by MTT assay.

Results: Detail phytochemical analysis reflected successful preparation of tannin-less MF of rhizomes. Different types of analytical techniques first time proved the proved the presence of various types of phytochemicals in this plant. On MTT assay, it was found that MF has anti-cancer property against NSCLC-A549 cell line with IC value, 306.51 μg/ml.

Conclusion: MF contains different phytochemicals like iridoids, flavonoids, steroid glycosides and also strigalactones; which cumulatively exert anti-cancer effect on A549. Appearance of all these compounds is significant in chemotaxonomic surveillance of this rare plant and specially, strigalactones can be proved important in establishing their parasitism with host plant.