Premise: Microsatellite markers were developed for the perennial herb (Lamiaceae), a species representative of European dry grasslands. The development of microsatellite markers is needed for genetic and phylogeographical studies of species from the genus .

Methods And Results: We used low-coverage Illumina sequencing to identify microsatellite loci. Based on these data, we have developed 18 polymorphic microsatellite markers with the number of alleles per locus ranging from two to 15. The levels of observed and expected heterozygosity ranged from 0.05 to 0.95 and from 0.05 to 0.89, respectively. The majority of the markers successfully cross-amplified in other species.

Conclusions: The markers were shown to be suitable for population genetic and phylogeographic studies in as well as in related species (, , , , , and ) and will be used in the broader context to trace the origins of European dry grasslands.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6976894PMC
http://dx.doi.org/10.1002/aps3.11316DOI Listing

Publication Analysis

Top Keywords

microsatellite markers
16
development microsatellite
8
european dry
8
dry grasslands
8
markers
6
markers premise
4
microsatellite
4
premise microsatellite
4
markers developed
4
developed perennial
4

Similar Publications

A variant W chromosome in Centromochlus heckelii (Siluriformes, Auchenipteridae) and the role of repeated DNA in its heteromorphism.

Genet Mol Biol

January 2025

Instituto Nacional de Pesquisas da Amazônia, Programa de Pós-Graduação em Genética, Conservação e Biologia Evolutiva (PPG GCBEv), Manaus, AM, Brazil.

Centromochlus heckelii has the lowest diploid chromosome number (2n = 46) and the only described heteromorphic sex chromosome system in Auchenipteridae. This study presents a population of C. heckelii from the Central Amazon basin with subtle variations in the karyotype composition and a variant W chromosome with distinct morphology and increased C-positive heterochromatin content.

View Article and Find Full Text PDF

Identification of chromosomal abnormalities is an important issue in animal breeding and veterinary medicine. Routine cytogenetic diagnosis of domestic animals began in the 1960s with the aim of identifying carriers of centric fusion between chromosome 1 and 29 in cattle. In the 1970s, chromosome banding techniques were introduced, and in the 1980s, the first cytogenomic techniques, based on the development of locus- and chromosome-specific probes, were used.

View Article and Find Full Text PDF

SSR marker-based genetic diversity and structure analyses of var. from different populations.

PeerJ

January 2025

Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica, Nanning, Guangxi, China.

Background: var. is a variety in the section of the genus of the family Theaceae which is native to Fangchenggang, Guangxi, China. To date, the genetic diversity and structure of this variety remains to be understood.

View Article and Find Full Text PDF

Enhancing trace DNA profile recovery in forensic casework using the amplicon RX post-PCR clean-up kit.

Sci Rep

January 2025

The Biology and DNA Section, General Department of Forensic Science and Criminology, Dubai Police General Head Quarters, Dubai, United Arab Emirates.

This study evaluated the effectiveness of the amplicon RX post-PCR clean-up kit in enhancing trace DNA profile recovery from forensic casework samples amplified using the GlobalFiler PCR amplification kit. The impact of post-PCR clean-up on allele recovery and signal intensity was assessed in both trace casework samples and control samples across a range of DNA concentrations. The results showed that the amplicon RX method significantly improved allele recovery compared to the 29-cycle protocol (p = 8.

View Article and Find Full Text PDF

Krait2: a versatile software for microsatellite investigation, visualization and marker development.

BMC Genomics

January 2025

Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Sichuan Province and Ministry of Education, Southwest Minzu University, Chengdu, 610225, China.

Background: Microsatellites are highly polymorphic repeat sequences ubiquitously interspersed throughout almost all genomes which are widely used as powerful molecular markers in diverse fields. Microsatellite expansions play pivotal roles in gene expression regulation and are implicated in various neurological diseases and cancers. Although much effort has been devoted to developing efficient tools for microsatellite identification, there is still a lack of a powerful tool for large-scale microsatellite analysis.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!