An aptasensor based on MIL-53(Al)@CdTe was designed for multiple determination of Hg and Pb by electrochemiluminescence (ECL). Upon the recognition of Hg, aptamer 2-AuNPs form hairpin structures and are removed from the electrode. While in the presence of Pb, aptamer 1-PtNPs capture the target ions and form G-quadruplexes, and then bring PtNPs close enough to CdTe QDs to produce ECL resonance energy transfer. Upon aptamer interaction with Hg and Pb, decreased ECL intensity was observed due to enhanced resonance energy transfer (ERET) and attenuated surface plasmon resonance (SPR). The ECL intensity difference (ΔECL) could therefore be used to detect heavy-metal ions with detection limits of 4.1 × 10 M (path 1, Hg), 3.7 × 10 M (path 2, Pb), and 2.4 × 10 M (path 3, Pb). The aptasensor could also be used for detecting Hg and Pb in fish and shrimp samples with good recoveries.
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