Background: Inflammation and pain, mainly induced by the prostaglandins synthesized by the cyclooxygenase enzymes, may cause distress. To overcome this unpleasant stress in a safer manner, numerous natural molecules are proven for modulating the COX enzymes. Epicatechin and daidzein are two bioactive natural compounds present in horsegram, a legume known for its medicinal properties.
Objective: The present study aims at evaluating the potential of horsegram, and some of its bioactive molecules, to be used as an anti-inflammatory and analgesic agent mediated by the inhibition of COX enzymes, which can be recommended as a substitute for chemically synthesized NSAIDs.
Methods: The present work involved the quantification of epicatechin and daidzein present in horsegram seeds. The COX enzyme inhibitory nature of epicatechin and daidzein was tested using in silico docking analysis with Autodock software and was further confirmed by in vitro COX inhibitory biochemical assays. Furthermore, the anti-inflammatory and analgesic activities of the horsegram seeds were evaluated in animal experiments.
Results: Horsegram seeds contain 158.1 microgram/g and 6.51 microgram/g of epicatechin and daidzein respectively. The docking studies reveal that both the bioactive molecules exhibit better binding efficiency with COX-2 protein as compared to COX-1. Hence, in vitro COX-2 inhibitory assay was performed for epicatechin, daidzein and compared with known analgesic agent diclofenac which revealed a pronounced dose dependent inhibitory activity. Furthermore, the analgesic and anti-inflammatory activity of horsegram in experimental animals exhibited a dose dependent effect which might be due to the presence of the bioactive compounds such as epicatechin and daidzein.
Conclusion: The results suggest that epicatechin and daidzein present in horsegram are potent cyclooxygenase inhibitors and thus would be helpful in the management of inflammation and pain.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.2174/1386207323666200127114551 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Unraveling the bioactive constituents of Typha elephantina: A comprehensive phytochemical analysis by tandem mass spectrometry.
PLoS One
December 2024
Department of Chemistry, Quaid-i-Azam University Islamabad, Islamabad, Pakistan.
Phytochemicals derived from plants have gained significant attention in recent years due to their diverse therapeutic properties. Typha elephantina is an aquatic plant having ameliorative characteristics like antioxidant, anti-inflammatory and analgesic etc. This research aims to conduct a comprehensive phytochemical investigation by Tandem mass spectrometry on the aerial parts and roots of Typha elephantina with a focus on identifying and characterizing the bioactive compounds present in it.
View Article and Find Full Text PDFBlack soybean extract inhibits rat mammary carcinogenesis through BRCA1 and TNF-α expression: and study.
Open Vet J
October 2024
Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kota Bharu, Kelantan, 16100, Malaysia.
Article Synopsis
- - Mammary gland carcinoma is a serious cancer caused by the carcinogen DMBA, which damages DNA, while flavonoids in black soybean possess anti-cancer properties.
- - A study tested the effects of black soybean extract on cancer in rats, using different dosages and measuring their impact on cancer-related proteins BRCA1 and TNF-α.
- - Results showed that black soybean extract is non-toxic and effectively inhibits cancer progression, with the best dosage being 200 mg/kg body weight, likely due to components like catechin and genistein.
Identification of the chemical composition of distiller's grain polyphenols and their effects on the fecal microbial community structure.
Food Chem X
December 2023
College of Liquor and Food Engineering, Key Laboratory of Fermentation Engineering and Biological Pharmacy of Guizhou Province, Guizhou University, Guiyang, Guizhou 550025, China.
Distiller grains are the main by-products of production and are usually discarded, ignoring their abundant functional phytochemicals. The free and bound polyphenols from distiller grains were extracted and their potential effect on modulating fecal microbiota was investigated using fecal fermentation. The results showed that 34 polyphenols were quantified from distiller grains.
View Article and Find Full Text PDFDeep eutectic solvents as an alternative for extraction of flavonoids from soybean (Glycine max (L) Merrill) and okara: An experimental and computational approach based on COSMO-SAC model.
Food Res Int
November 2023
Laboratory of Extraction, Applied Thermodynamics and Equilibrium - EXTRAE, School of Food Engineering, University of Campinas, 80 Monteiro Lobato Street, 13083-062 Campinas, Brazil. Electronic address:
In this study, different Deep Eutectic Solvents based on choline chloride ([Ch]Cl) with carboxylic acids, sugars, and glycerol, were investigated as alternative solvents for the extraction of flavonoids from soybean and okara. Initially, the COSMO-SAC was investigated as a tool in solvent screening for the extraction of flavonoids. Experimental validation was performed using total flavonoid analysis with the solvents that showed greater interaction with the solutes.
View Article and Find Full Text PDFProtective Activity of bv. Strain 33504-Mat209 against Alfalfa Mosaic Virus Infection in Faba Bean Plants.
Plants (Basel)
July 2023
Agriculture Microbiology Department, National Research Centre, Cairo 12622, Egypt.
The application of spp., nitrogen-fixing plant growth-promoting rhizobacteria, as biocontrol agents to enhance systemic disease resistance against plant viral infections is a promising approach towards achieving sustainable and eco-friendly agriculture. However, their potential as antivirals and biocontrol agents is less studied.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!