AI Article Synopsis
- - This study introduces a simple one-step method for creating antibody phage display libraries using type IIs restriction enzymes, allowing easy cloning of single chain variable fragments (scFv) without disrupting the DNA sequence.
- - The authors successfully isolate antigen-specific antibodies from immunized chickens in just three selection rounds, indicating the efficiency of their cloning approach.
- - The technique is also shown to work effectively for constructing VHH single-domain antibody libraries from immunized llamas, proving its versatility.
Article Abstract
In this work we present a one-step cloning approach for the establishment of antibody phage display libraries relying on type IIs restriction enzymes. We show that single chain variable fragment (scFv) libraries with adequate qualities can readily be cloned in a 'scar-less' manner and that the isolation of antigen-specific antibodies from immunized chickens is feasible within three selection rounds. Moreover, we demonstrate the general applicability of this method by rapidly constructing and panning VHH single domain antibody phage display libraries from immunized Llama repertoires.
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| http://dx.doi.org/10.1007/s12033-020-00236-0 | DOI Listing |
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