Lysobacter prati sp. nov., isolated from a plateau meadow sample.

A novel proteobacterial strain designated SYSU H10001 was isolated from a soil sample collected from plateau meadow in Hongyuan county, Sichuan province, south-western China. The taxonomic position of the strain was investigated using a polyphasic approach. On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU H10001 was most closely related to Lysobacter soli KCTC 22011 (98.6%, sequence similarity) and Lysobacter panacisoli JCM 19212 (98.2%). The prediction result of secondary metabolites based on genome shown that the strain SYSU H10001 contained 3 clusters of bacteriocins, 1 cluster of non-ribosomal peptide synthetase, 1 cluster of type 1 polyketide synthase and 1 cluster of arylpolyene. In addition, the major isoprenoid quinone was Q-8 and the major fatty acids were identified as iso-C, iso-C and Summed feature 9. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and three unidentified phospholipids. The genomic DNA G + C content of strain SYSU H10001 was 66.5% (genome). On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU H10001 represents a novel species of the genus Lysobacter, for which the name Lysobacter prati sp. nov. is proposed. The type strain is SYSU H10001 (= KCTC 72062 = CGMCC 1.16662).