Using the Oocyte Toolbox.

Cold Spring Harb Protoc

Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Box G-L268, Providence, Rhode Island 02912.

Published: April 2020

The oocyte is a unique model system, allowing both the study of complex biological processes within a cellular context through expression of exogenous mRNAs and proteins, and the study of the cell, molecular, and developmental biology of the oocyte itself. During oogenesis, oocytes grow dramatically in size, with a mature oocyte having a diameter of ∼1.3 mm, and become highly polarized, localizing many mRNAs and proteins. Thus, the mature oocyte is a repository of maternal mRNAs and proteins that will direct early embryogenesis prior to zygotic genome transcription. Importantly, the oocyte also has the capacity to translate exogenous microinjected RNAs, which has enabled breakthroughs in a wide range of areas including cell biology, developmental biology, molecular biology, and physiology. This introduction outlines how oocytes can be used to study a variety of important biological questions.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7250317PMC
http://dx.doi.org/10.1101/pdb.top095844DOI Listing

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