can measure the activity of the enzymes that remodel the cell wall to ensure that the levels of activity are 'just right'.
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http://dx.doi.org/10.7554/eLife.54244 | DOI Listing |
Biomolecules
July 2023
Department of Chemistry, Faculty of Science and Technology, UiT the Arctic University of Norway, 9019 Tromsø, Norway.
Antimicrobial peptides (AMPs) are generally membrane-active compounds that physically disrupt bacterial membranes. Despite extensive research, the precise mode of action of AMPs is still a topic of great debate. This work demonstrates that the initial interaction between the Gram-negative and AMPs is driven by lipopolysaccharides (LPS) that act as kinetic barriers for the binding of AMPs to the bacterial membrane.
View Article and Find Full Text PDFAdv Sci (Weinh)
April 2022
Carbone Cancer Center, University of Wisconsin-Madison, Madison, WI, 53705, USA.
Oxygen levels in vivo are autonomously regulated by a supply-demand balance, which can be altered in disease states. However, the oxygen levels of in vitro cell culture systems, particularly microscale cell culture, are typically dominated by either supply or demand. Further, the oxygen microenvironment in these systems is rarely monitored or reported.
View Article and Find Full Text PDFAntimicrob Agents Chemother
July 2020
Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia, USA
Elife
January 2020
College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, Pomona, United States.
can measure the activity of the enzymes that remodel the cell wall to ensure that the levels of activity are 'just right'.
View Article and Find Full Text PDFMicrobiology (Reading)
October 2019
School of Applied Sciences, Abertay University, Dundee DD1 1HG, UK.
In radiating populations of SBW25, adaptive wrinkly spreader (WS) mutants are able to gain access to the air-liquid (A-L) interface of static liquid microcosms and achieve a significant competitive fitness advantage over other non-biofilm-forming competitors. Aerotaxis and flagella-based swimming allows SBW25 cells to move into the high-O region located at the top of the liquid column and maintain their position by countering the effects of random cell diffusion, convection and disturbance (i.e.
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