Detection of , and spp. in Adult Black-Legged Ticks () from Pennsylvania, United States, with a Luminex Multiplex Bead Assay.

, the black-legged tick, harbors multiple organisms and transmits several pathogens to animals and humans. To determine the presence of tick-borne microorganisms carried by in Pennsylvania, 299 adult ticks were collected from across the state and tested with a multiplex bead panel targeting 20 microorganisms. The Luminex bead-based xMAP MultiFLEX Mega Tick Panel detected microorganisms in these ticks, including spp. (1.7%), spp. (45.8%), spp. (16.1%), and spp. (22.1%) at the genera level and identified (1.7%), (0.7%), stricto (45.5%), (0.3%), and (0.7%) at the species level. spp. reactivity was found to be due to , and spp. reactivity was mainly due to rickettsial endosymbionts.