Background: Plants have been suggested as safest source of therapeutic agents, with multi targeted mode of action and least side effects. commonly known as Guduchi in India, is one of the most highly valued herbs in Ayurvedic medicine. It possesses potential anti-cancer, anti-inflammatory, hepatoprotective, anti-diabetic, immune-stimulatory and various other beneficial activities.
Purpose: The present study was aimed to investigate the differentiation inducing potential of chloroform and hexane extracts of using U87MG glioblastoma and IMR-32 neuroblastoma cell lines as model system.
Results: Chloroform (Chl-TCE) and hexane (Hex-TCE) extracts significantly reduced the rate of proliferation and induced cell differentiation as evidenced by MTT assay and immunostaining for GFAP and MAP-2 in glioblastoma and neuroblastoma, respectively. Further these extracts increased the expression of stress markers HSP70 and Mortalin and induced senescence. Chloroform and hexane extracts also inhibited the migration of U87MG glioblastoma and IMR-32 neuroblastoma as indicated by wound scratch assay and supported by reduced expression of NCAM. Furthermore these extracts are not toxic to normal cells as they showed no inhibitory effects on primary astrocytic and neuronal cultures.
Conclusions: The present study suggests that chloroform and hexane extracts of retard the rate of proliferation, induce differentiation and inhibit migration of human glioblastomas and neuroblastomas, thus may act as potential phytotherapeutic intervention in treatment of neural cancers.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6894632 | PMC |
| http://dx.doi.org/10.5214/ans.0972.7531.260104 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Investigating the Protective Effects of Luteolin and Gallic Acid from Luffa acutangula L. (Roxb.) amara (Roxb.) C. B. Clarke. Fruit Pericarp Against Alcohol-Induced Liver Toxicity: Extraction, Bioactivity-Guided Fractionation, Molecular Docking, and Dynamics Studies.
J Ethnopharmacol
January 2025
Department of Pharmacognosy, Goa College of Pharmacy, Panaji, Goa, 403 001, India. Electronic address:
Ethnopharmacological Relevance: Luffa acutangula var. amara (Roxb.) C.
View Article and Find Full Text PDFEvaluation of the Therapeutic Potential of the Methanolic Extract and Fractions of Datura stramonium in Paracetamol-intoxicated Rabbits.
Curr Pharm Des
January 2025
Department of Horticulture and Life Science, Yeungnam University, Republic of Korea.
Introduction: Datura stramonium (DS) possesses strong medicinal and therapeutic potential but has been rarely evaluated in this context.
Methods: The present study was intended to evaluate the antioxidant, hepatoprotective, and nephroprotective potential of the crude methanolic leaf extract and ethyl acetate, chloroform, n-hexane, and aqueous fractions of DS in paracetamol-intoxicated rabbits. Paracetamol (2 g/Kg BW) was applied to induce liver and kidney injury in rabbits while the methanolic extract and fractions of DS were applied in the dose range of 150 mg/Kg to 300 mg/Kg body weight for 21 days.
Development and validation of a rapid and accurate ultra performance liquid chromatography-photodiode array method for concurrent quantification of thirty-two polyphenols in edible fruit of Cordia myxa Linn.
J Chromatogr A
December 2024
Chemical Technology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur (Himachal Pradesh), 176061, India; Academy of Scientific and Innovative Research, (AcSIR), Ghaziabad, 201002, India. Electronic address:
The aim of current work was to develop a novel, simple, sensitive, and reliable method for screening and quantification of thirty-two polyphenol compounds from Cordia myxa (C. myxa) using Ultra Performance Liquid Chromatography Photodiode Array detector (UPLC-PDA). With the help of the quaternary solvent manager and a comparison study of seven different columns packed with silica particles that are less than two micron thick (1.
View Article and Find Full Text PDFPhytochemical analysis and biological activities of solvent extracts and silver nanoparticles obtained from Woodwardia unigemmata (Makino) Nakai.
PLoS One
January 2025
Department of Pharmaceutics, College of Pharmacy, King Khalid University, Abha, Saudi Arabia.
Multidrug resistant bacteria are causing health problems and economic burden worldwide; alternative treatment options such as natural products and nanoparticles have attained great attention recently. Therefore, we aimed to determine the phytochemicals, antibacterial potential, and anticancer activity of W. unigemmata.
View Article and Find Full Text PDFA Protocol for GC-MS Profiling of Chiral Secondary Amino Acids.
Methods Mol Biol
January 2025
Laboratory of Analytical Biochemistry & Metabolomics, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic.
A simple analytical workflow is described for gas chromatographic-mass spectrometric (GC-MS)-based chiral profiling of secondary amino acids (AAs) in biological matrices. The sample preparation is carried out directly in aqueous biological sample extracts and involves in situ heptafluorobutyl chloroformate (HFBCF) derivatization-liquid-liquid microextraction of nonpolar products into hexane phase followed by subsequent formation of the corresponding methylamides from the HFB esters by direct treatment with methylamine reagent solution. The (O, N) HFB-butoxycarbonyl-methylamide AA products (HFBOC-MA) are separated on a Chirasil-L-Val capillary column and quantitatively measured by GC-MS operated in selected ion monitoring (SIM) mode.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!