Genome-wide characterization of RNA populations in early flowering plant embryos can yield insights into the gene regulatory processes functioning during this formative phase of development. However, early embryonic transcriptomes are technically challenging to profile because of the low amount of RNA obtainable and potential RNA contamination from surrounding nonembryonic tissues. Here we provide a detailed protocol for collecting early Arabidopsis thaliana (Arabidopsis) embryos, generating mRNA sequencing (mRNA-seq) libraries, and basic data processing and quality controls of the resulting mRNA-seq data.
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Article Synopsis
- Artemia is a microcrustacean known for its embryos' ability to withstand extreme conditions, thanks in part to a protective protein called artemin.
- Researchers cloned the artemin gene and introduced it into Arabidopsis thaliana to study its impact on heat stress tolerance.
- Results showed that transgenic plants with the artemin gene had improved germination rates, root growth, and stress-related gene expression, indicating enhanced heat stress resistance compared to wild-type plants.*
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