AI Article Synopsis
- MicroRNAs (miRNAs) play a key role in regulating gene expression, especially in the context of probiotics like ssp BB12, which influence immune responses by modulating cytokine production and antigen presentation.
- Researchers conducted an analysis using RNAHybrid software to identify target genes of specific miRNAs in monocyte-derived dendritic cells (moDCs) stimulated with BB12, discovering 23 genes linked to 19 miRNAs, with particular emphasis on miR-30b-3p, miR-671-5p, and miR-9858-5p.
- The study found that these miRNAs can down-regulate costimulatory molecules SLA-DR and CD80 in BB12-stimulated moDCs,
Article Abstract
MicroRNAs (miRNAs) mediate the regulation of gene expression. Several reports indicate that probiotics induce miRNA-mediated immunomodulation at different levels, such as cytokine production and the up-regulation of several markers related to antigen presentation in antigen-presenting cells. The objective of this work was to identify target genes of miRNAs that are involved in the processing and presentation of antigens in monocyte-derived dendritic cells (moDCs) stimulated with the probiotic ssp BB12 (BB12). First, an in silico prediction analysis for a putative miRNA binding site within a given mRNA target was performed using RNAHybrid software with mature sequences of differentially expressed miRNAs retrieved from a Genbank data set that included BB12-stimulated and unstimulated porcine monocytes. From them, 23 genes resulted in targets of 19 miRNAs, highlighting miR-30b-3p, miR-671-5p, and miR-9858-5p, whose targets were costimulatory molecules, and were overexpressed ( < 0.05) in BB12-stimulated moDCs. The analysis of moDCs showed that the percentage of cells expressing SLA-DRCD80 decreased significantly ( = 0.0081) in BB12-stimulated moDCs; interleukin (IL)-10 production was unchanged at 6 h but increased after 24 h of culture in the presence of BB12 ( < 0.001). In summary, our results suggest that SLA-DR and CD80 can be down-regulated by miRNAs miR-30b-3p, miR-671-5p, and miR-9858-5p, while miR-671-5p targets IL-10.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037397 | PMC |
| http://dx.doi.org/10.3390/ijms21030687 | DOI Listing |
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