sp. nov., a glutamate producing bacterium isolated from soil and its complete genome-based analysis.

Strain N24 was isolated from soil contaminated with starling's feces collected from Roi-Et province, Thailand. Cells of N24 were Gram-stain-positive rods, aerobic and non-spore-forming. N24 was positive for catalase, urease, citrate utilization, nitrate reduction and Methyl Red (MR) test but negative for oxidase, casein, gelatin liquefaction, tyrosine, Voges-Proskauer (VP) reaction and starch hydrolysis. -diaminopimelic acid, rhamnose, ribose, arabinose and galactose were detected in its whole-cell hydrolysates. The results of the 16S rRNA gene sequence analysis indicated that N24 represented a member of the genus . N24 was closely related to ATCC 13032, with 99.0 % 16S rRNA gene sequence similarity. According to results obtained using DNA-DNA hybridization approaches, N24 showed highest DNA-DNA relatedness (27.6 %) and average nucleotide identity (84.1 %) to ATCC 13032. The DNA G+C content of N24 was 51.8 mol% (genome based). The major cellular fatty acids of N24 were C, and Cω9. N24 had the nine isoprenes unit, MK-9(H) as the predominant menaquinone. The predominant polar lipids were phosphatidylglycerol, phosphatidylinositol and diphosphatidylglycerol. Mycolic acids were also present. According to the complete genome sequence data, strain N24 and ATCC 13032 are close phylogenetic neighbours, but have different genome characteristics. On the basis of the results of the genotypic and genomic studies and phenotypic characteristics including chemotaxonomy, strain N24 should be classified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is N24 (TBRC 5845=NBRC 113465).