Interleukin 2 (IL-2) treatment is associated with proteinuria. A conditionally immortalized human podocyte cell line was used to investigate expression of the podocyte specific marker podocin, IL-2R alpha (IL-2Rα), apoptosis marker Bax, and autophagy markers LC3I AND LC3II, determined by quantitative immunoblotting, following 24, 48, and 72 hours of IL-2 stimulation, comparing them to unstimulated cells. Podocin was expressed at all time points. IL-2Rα expression was increased after 24 and 72 hrs ( 0.0014, 0.0139) and decreased after 48 hours ( 0.0445). Bax, LC3I, and LC3II were increased after 24 hrs ( 0.0094, 0.0016, 0.0004) and 48 hrs ( 0.0072, 0.0024, 0.0087). Human podocytes express the IL-2R and activation results in increased autophagy and apoptosis.
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