Overexpression of AmpC Promotes Bacteriophage Lysis of Ampicillin-Resistant .

Infections caused by antibiotic-resistant are a threat to human and animal health globally. Phage therapy has made great progress for the treatment of drug-resistant infections, but it is still unclear whether resistance to antibiotics could change the lysis ability of phages. In this study, we demonstrate that over expression of AmpC, an important β-lactamase for ampicillin resistance, promotes lysis of by phage utilizing OmpA as a receptor. strains expressing more AmpC showed higher levels of OmpA, an outer membrane protein known to serve as a receptor for T-even phages, which resulted in increased adsorption and lysis by the phage tested in this study. These data demonstrate that increased ampicillin resistance can increase the sensitivity of to some lytic phage, which provides evidence for the feasibility of synergistic application of phage and antibiotics.