AI Article Synopsis
- The study explores the production of cultured human platelets from CD34 progenitors for transfusion, aiming to create a safer alternative to traditional platelet sources.
- These cultured platelets showed normal morphology and functionality, successfully aggregating in response to specific triggers and maintaining a similar survival rate as native platelets in a mouse model.
- The findings suggest that these cultured platelets effectively reduce blood loss in bleeding scenarios, making them a promising candidate for future clinical trials in transfusion medicine.
Article Abstract
The in vitro production of blood platelets for transfusion purposes is an important goal in the context of a sustained demand for controlled products free of infectious, immune and inflammatory risks. The aim of this study was to characterize human platelets derived from CD34 progenitors and to evaluate their hemostatic properties. These cultured platelets exhibited a typical discoid morphology despite an enlarged size and expressed normal levels of the major surface glycoproteins. They aggregated in response to ADP and a thrombin receptor agonist peptide (TRAP). After infusion into NSG mice, cultured and native platelets circulated with a similar 24 h half-life. Notably, the level of circulating cultured platelets remained constant during the first two hours following infusion. During this period of time their size decreased to reach normal values, probably due to their remodeling in the pulmonary circulation, as evidenced by the presence of numerous twisted platelet elements in the lungs. Finally, cultured platelets were capable of limiting blood loss in a bleeding assay performed in thrombocytopenic mice. In conclusion, we show here that cultured platelets derived from human CD34 cells display the properties required for use in transfusion, opening the way to clinical trials.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6976668 | PMC |
| http://dx.doi.org/10.1038/s41598-020-57754-9 | DOI Listing |
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