AI Article Synopsis
Article Abstract
This study was to investigate the effects of microRNA-21 (miR-21) on ox-LDL-induced HUVECs apoptosis. MTT assay was performed to evaluate the proliferation of HUVECs. Quantitative RT-PCR was conducted to quantify the expression of miR-21. Western blotting was used to determine protein expression. Annexin V/propidium iodide double staining was adopted to detect cell apoptosis. We found that ox-LD significantly induced HUVECs apoptosis and reduced miR-21 expression. MiR-21 mimic attenuated the apoptosis of HUVECs under ox-LDL treatment compared with the NC groups while miR-21 inhibitors promoted that of HUVECs. MiR-21 directly targeted PDCD4 in HUVECs. Moreover, miR-21 significantly regulated the downstream apoptotic proteins like bax, bad, bcl-2 and caspase3, meanwhile it enhanced the phosphorylation of ERK.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6965965 | PMC |
Publication Analysis
Top Keywords
Similar Publications
miR-1233-3p Inhibits Angiopoietin-1-Induced Endothelial Cell Survival, Migration, and Differentiation.
Cells
January 2025
Meakins-Christie Laboratories, Department of Medicine, McGill University, Montreal, QC H4A 3J1, Canada.
Angiopoietin-1 (Ang-1) and its receptor Tie-2 promote vascular integrity and angiogenesis. MicroRNAs (miRNAs) are involved in the regulation of many cellular functions, including endothelial cell (EC) survival, proliferation, and differentiation. Several reports indicate that these effects of miRNAs on EC functions are mediated through the modulation of angiogenesis factor signaling including that of vascular endothelial growth factor (VEGF).
View Article and Find Full Text PDFAnti-angiogenic effects of cationic zinc (II) phthalocyanine derivatives through photodynamic therapy.
Sci Rep
January 2025
TUBITAK Marmara Research Center, Climate Change and Life Sciences, Biotechnology Research Group, 41470, Kocaeli, Turkey.
In this study, the in vitro photodynamic therapy (PDT) activity of two zinc phthalocyanines (ZnPc1 and ZnPc2) was systematically examined in human umbilical vein endothelial cells, focusing on PDT-induced cytotoxicity, reactive oxygen species (ROS) generation, and inhibition of angiogenic processes. Both the ZnPcs demonstrated minimal cytotoxicity in the absence of light, confirming their safety as photosensitizers. ZnPc-PDT led to significant cell death via apoptosis.
View Article and Find Full Text PDFMOTS-c mimics remote ischemic preconditioning in protecting against lung ischemia-reperfusion injury by alleviating endothelial barrier dysfunction.
Free Radic Biol Med
January 2025
Department of Anesthesiology, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi People's Hospital, Wuxi Medical Center, Nanjing Medical University, Wuxi, Jiangsu, 214023, China. Electronic address:
Remote ischemic preconditioning (RIPC) induces the expression of unidentified protective cytokines that mitigate lung ischemia-reperfusion injury (LIRI). This study hypothesizes that MOTS-c, a mitokine with potent protective effects against mitochondrial damage, contributes to RIPC-mediated protection by alleviating endothelial barrier dysfunction. In human lung transplantation patients, serum levels of MOTS-c significantly decreased following IR injury but were markedly increased when RIPC was performed prior to transplantation.
View Article and Find Full Text PDFA novel and high-performance tumor inhibitor of La, N co-doped carbon dots for U251 and LN229 cells.
Colloids Surf B Biointerfaces
January 2025
Department of Neurosurgery, The Affiliated Ganzhou Hospital, Jiangxi Medical College, Nanchang University, Ganzhou 341000, China. Electronic address:
To address the medical challenges posed by glioblastoma, a novel and high-performance tumor inhibitor (La@FA-CDs) composed of folic acid and lanthanum nitrate hexahydrate, was successfully synthesized and demonstrated effectiveness in inhibiting the growth of U251 and LN299 cells. The microstructure of La@FA-CDs was extensively analyzed by FTIR, UV-Vis, XPS, TEM, AFM NMR, and nanoparticle size analyzer. The optical and electrical properties of La@FA-CDs were characterized using a fluorescence spectrometer and a zeta potential analyzer.
View Article and Find Full Text PDFNovel Dehydroabietylamine C-Ring Schiff Base Derivatives: Synthesis, Antiproliferative activity, DNA binding and Molecular docking.
Chem Asian J
January 2025
College of Science, Nanjing Forestry University, Nanjing, Jiangsu, 210037, China.
A series of Dehydroabietylamine (DHAA) C-ring Schiff derivatives, L-L, were synthesized and their in vitro cytotoxic activity against the human tumor cell lines cervix HeLa, breast MCF-7, lung A549, liver HepG2, and the nonmalignant cell line umbilical vein HUVEC was investigated. Most of the compounds showed varying degrees of anticancer activity against HeLa cell lines while demonstrating lower toxicity to normal HUVEC cells compared to DHAA and doxorubicin (DOX), especially compound L, which not only enhanced the anticancer activity of DHAA, but also significantly reduced the toxicity to normal cells, achieving a selectivity index (SI) 118 times higher than that of DHAA and 245 times higher than that of DOX. In addition, Compound L induced apoptosis in HeLa cells in a dose-dependent manner, suppressed the expression of the anti-apoptotic protein Bcl-2, and arrested the cell cycle at the S phase.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!