The association of inhibitor of Growth 4 with its prognostic value in osteogenic sarcoma.

Background: Osteosarcoma is one of the first two cause of cancer-related death in children and young adolescents. Inhibitor of Growth 4 (ING4) is a member of the ING tumor suppressor family and play an important role in many cellular processes. The purpose of this study was to explore the correlation between expression and the prognosis of osteosarcoma patients.

Methods: mRNA and protein expressions in osteosarcoma and normal tissues were detected by quantitative real-time transcriptase polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC) method, respectively. Chi-square test was adopted to estimated the relationship of expression and clinical parameters of osteosarcoma patients. Besides, the overall survival of osteosarcoma patients was evaluated by Kaplan-Meier method. The potential of as a prognostic marker gene was addressed by Cox regression analysis.

Results: Down-regulated expression of mRNA and protein were observed in osteosarcoma tissues. expression was significantly associated with metastasis ( = 0.030) and recurrence ( = 0.008), but not other clinical features ( > 0.05). Cox regression analysis indicated that can be used as an independent prognotic biomarker for osteosarcoma, in univariate and multivariate analysis ( = 0.004, HR = 3.945, 95 % CI = 1.565-9.940; = 0.001, HR = 4.213, 95 % CI = 1.747-10.161).

Conclusion: Taken together, was down-regulated in osteosarcoma tissues. can act as an independent prognostic factor for osteosarcoma.