Dynamaic changes of proliferation and apoptosis in rat retina development.

Precise coordination of cell proliferation and apoptosis is essential for proper organ morphogenesis and function during mammalian development. The retina is a part of the central nervous system, with a function in capture and conversion of light particles into nerve signals. However, the dynamical changes of cell proliferation and apoptosis during the retinal development is unclear. Thus, this study was to observe the changes of proliferating cell nuclear antigen expression and apoptosis during the retina development in rats. Rats from different developmental stages (embryonic days 14-20 (E14-20), postnatal days 0-15 (P0-15) and adult (P36)) were used. The eyeballs were removed and fixed immediately in formalin. The tissues were embedded in paraffin. Sections were prepared for immunohistochemical staining and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL). PCNA expression appeared in large quantities in rat retina from E14 to P7, decreased obviously since P9, and could not be observed since P15. Apoptotic cells appeared in small quantities since E18. And then apoptotic cells dramatically increased during the retina development, and peaked at P7. As maturation continued, these cells decreased gradually. Apoptotic cells could not be found in the mature retina. Cell proliferation and apoptosis shows orderly and dynamic changes during the retina development, which is essential for the development of normal structure and function of the retina.