MiR-7684-5p leads to surgery-induced cognitive decline in mice probably through the downregulation of SorLA.

Background: Postoperative cognitive dysfunction is a postoperative severe complication caused by many factors. However, its specific pathogenesis remains unclear. MicroRNAs (miRNAs), which are involved in the pathogenesis of neurodegenerative diseases, may also affect POCD.

Methods: In this research, microarray technology was used to screen 26 miRNAs that had a differential expression in the hippocampus of mouse between the surgery group and control group. The qRT-PCR verification on the hippocampuses of 10 pairs of mouse testifies the high expression of miR-7684-5p in the surgery group (identical with the result of chip).

Results: Surgical trauma was found to induce the expression of miR-7684-5p with the accumulation of Aβ in the hippocampus. Furthermore, miR-7684-5p knockdown effectively reduced the levels of Aβ triggered by surgery, and attenuated hippocampal-dependent memory impairment. Moreover, we testify that sorLA is a target gene of miR-7684-5p through bioinformatics prediction and dual-luciferase report gene experiment.

Conclusions: Our data indicate that decreased postoperative cognitive function may be caused by the increased generation of Aβ by reducing sorLA expression. Our work implicates miR-7684-5p as a potential biomarker and a novel therapeutic target.