Here, we presented 11 cases with colistin-resistant infection and co-existence of OXA-48 and NDM-1 in the ST235 high-risk clone. The molecular analyses were performed by Sanger sequencing and RT-PCR. The eight patients (72.7%) had an invasive infection and three (27.3%) had colonization. The 30-day mortality rate was 87.5% (7/8). Three patients (37.5%, 3/8) received colistin therapy before isolation of . In the Multilocus sequence typing (MLST) analysis of 11 isolates, eight (72.7%) isolates belonged to ST235 clone. All isolates were NDM-1 positive, and nine isolates (81.8%) were found to be positive for both OXA-48 and NDM-1. Sequences of and revealed numerous insertions and deletions in all isolates. In 10 isolates and were found to be upregulated. In conclusion, the co-existence of OXA-48 and NDM-1 genes in colistin-resistant ST235 high-risk clone indicates the spread of carbapenemases in clinical isolates and highlights need of continuous surveillance for high-risk clones of .
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| http://dx.doi.org/10.1080/22221751.2020.1713025 | DOI Listing |
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