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Bone Marrow-Derived Mesenchymal Stromal Cells (MSCs) Modulate the Inflammatory Character of Alveolar Macrophages from Sarcoidosis Patients. | LitMetric

AI Article Synopsis

  • Sarcoidosis is a serious inflammatory disease mainly affecting the lungs and lymph nodes, and the study investigates the role of bone marrow-derived mesenchymal stromal cells (MSCs) in modifying macrophage behavior in patients with this condition.
  • Researchers co-cultured alveolar macrophages from sarcoidosis patients with MSCs and found that this interaction led to a shift towards an anti-inflammatory response, indicated by decreased TNF-α levels and increased IL-10 levels in most samples.
  • The study suggests that MSCs could be a potential therapeutic option for reducing lung inflammation in sarcoidosis patients, potentially decreasing their reliance on steroids.

Article Abstract

Sarcoidosis is a devastating inflammatory disease affecting many organs, especially the lungs and lymph nodes. Bone marrow-derived mesenchymal stromal cells (MSCs) can "reprogram" various types of macrophages towards an anti-inflammatory phenotype. We wanted to determine whether alveolar macrophages from sarcoidosis subjects behave similarly by mounting an anti-inflammatory response when co-cultured with MSCs. Fifteen sarcoidosis and eight control subjects underwent bronchoscopy and bronchoalveolar lavage (BAL). Unselected BAL cells (70-94% macrophages) were isolated and cultured with and without MSCs from healthy adults. Following stimulation of the cultured cells with lipopolysaccharide, the medium was removed to measure interleukin 10 and tumor necrosis factor alpha (IL-10 and TNF-α). In two additional sarcoidosis subjects, flow cytometry was used to study intracellular cytokines and surface markers associated with alveolar macrophages to confirm the results. Unselected BAL cells from sarcoidosis subjects co-cultured with MSCs showed a reduction in TNF-α (pro-inflammatory M1) and an increase in IL-10 (anti-inflammatory M2) in 9 of 11 samples studied. Control subject samples showed few, if any, differences in cytokine production. Unselected BAL cells from two additional patients analyzed by flow cytometry confirmed a switch towards an anti-inflammatory state (i.e., M1 to M2) after co-culture with MSCs. These results suggest that, similarly to other macrophages, alveolar macrophages also respond to MSC contacts by changing towards an anti-inflammatory phenotype. Based on our results, we hypothesize that mesenchymal stromal cells applied to the airways might alleviate lung inflammation and decrease steroid need in patients with sarcoidosis.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019909PMC
http://dx.doi.org/10.3390/jcm9010278DOI Listing

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