AI Article Synopsis

  • The study presents an advanced method using high-resolution mass spectrometry (HR-MS) to analyze changes in the composition of cerebellar gangliosides during development and aging.
  • By comparing gangliosides in fetal versus aged cerebellum using Orbitrap MS with nanoelectrospray ionization, researchers identified 159 ions from 120 different species, significantly expanding the understanding of ganglioside diversity in human cerebellum.
  • The findings revealed age- and development-specific ganglioside profiles, with some structural features remaining unchanged, suggesting potential for using gangliosides as biomarkers in cerebellar health studies.

Article Abstract

We have developed here a superior approach based on high-resolution (HR) mass spectrometry (MS) for monitoring the changes occurring with development and aging in the composition and structure of cerebellar gangliosidome. The experiments were focused on the comparative screening and structural analysis of gangliosides expressed in fetal and aged cerebellum by Orbitrap MS with nanoelectrospray ionization (nanoESI) in the negative ion mode. The employed ultrahigh-resolution MS platform allowed the discrimination, without the need of previous separation, of 159 ions corresponding to 120 distinct species in the native ganglioside mixtures from fetal and aged cerebellar biopsies, many more than detected before, when MS platforms of lower resolution were employed. A number of gangliosides, in particular polysialylated belonging to GT, GQ, GP, and GS classes, modified by O-fucosylation, O-acetylation, or CH COO were discovered here, for the first time in human cerebellum. These components, found differently expressed in fetal and aged tissues, indicated that the ganglioside profile in cerebellum is development stage- and age-specific. Following the fragmentation analysis by high-energy collision-induced dissociation (HCD) tandem MS (MS/MS), we have also observed that the intimate structure of certain compounds has not changed during the development and aging of the brain, an aspect which could open new directions in the investigation of ganglioside biomarkers in cerebellar tissue.

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http://dx.doi.org/10.1002/jms.4502DOI Listing

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