Genetic differentiation and phylogenetic relationship of 11 Asian Sisorinae genera (Siluriformes: Sisoridae) with new record of .

Studies on systematics have been largely restricted to morphological data with few studies on examination of phylogenetic relations. However, no study has been done to evaluate genetic distance of the genera under sub-family and detailed phylogenetic relations within it. We used nuclear recombination activating 2 () gene and mitochondrial cytochrome c oxidase I () gene from 64 species to examine genetic differentiation and phylogenetic relationships within 11 Asian genera. The range of interspecies K2P distance for was 0-0.061 and was 0-0.204. Phylogenetic analysis based on maximum likelihood (ML) and Bayesian (BI) approaches for each locus individually and for the concatenated and sequences revealed three major subclades viz. Bagariini, Sisorini and Erethistini under subfamily Sisorinae. The analysis based on gene showed ((Sisorini, Bagariini), Erethistini) relationship. 2 and combined and showed ((Sisorini, Erethistini), Bagariini) relationship. Combined 2 and analyses resulted into better resolved trees with a good bootstrap support. In this study, new record of (Erethistini) has been documented based on 13 specimens collected from Torsa River, Jaldapara, Alipurduar district, West Bengal, India (26°43'44.66″ N and 89°19'32.34″ E), extending its distribution range in Brahmaputra drainage, India. The genetic distance between the new record and the reported (holotype: UMMZ 244867) was 0.00 at both and locus and it was further grouped with Type specimen (holotype: UMMZ 244867) with 100% bootstrap support. This report gives additional information on occurrence of the species , along with discussion on morphometric, meristic and molecular ( and gene) data.