AI Article Synopsis
- Researchers have developed a new HCN CPMAS probe that increases sensitivity in MAS NMR experiments by 3-4 times, allowing for better analysis of complex biological systems.
- This probe operates with its sample coil and electronics at cryogenic temperatures while maintaining the sample at normal temperatures, which enhances data collection for 2D and 3D experiments.
- The technology has shown promising results with difficult samples, such as protein assemblies and prion fibrils, suggesting it could greatly benefit studies involving low-sensitivity biological systems.
Article Abstract
Despite breakthroughs in MAS NMR hardware and experimental methodologies, sensitivity remains a major challenge for large and complex biological systems. Here, we report that 3-4 fold higher sensitivities can be obtained in heteronuclear-detected experiments, using a novel HCN CPMAS probe, where the sample coil and the electronics operate at cryogenic temperatures, while the sample is maintained at ambient temperatures (BioSolids CryoProbe™). Such intensity enhancements permit recording 2D and 3D experiments that are otherwise time-prohibitive, such as 2D N-N proton-driven spin diffusion and N-C double cross polarization to natural abundance carbon experiments. The benefits of CPMAS CryoProbe-based experiments are illustrated for assemblies of kinesin Kif5b with microtubules, HIV-1 capsid protein assemblies, and fibrils of human Y145Stop and fungal HET-s prion proteins - demanding systems for conventional MAS solid-state NMR and excellent reference systems in terms of spectral quality. We envision that this probe technology will be beneficial for a wide range of applications, especially for biological systems suffering from low intrinsic sensitivity and at physiological temperatures.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7060763 | PMC |
| http://dx.doi.org/10.1016/j.jmr.2019.106680 | DOI Listing |
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