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Correction to: Vitrification within a nanoliter volume: oocyte and embryo cryopreservation within a 3D photopolymerized device.
J Assist Reprod Genet
October 2022
Australian Research Council (ARC) Centre of Excellence for Nanoscale BioPhotonics (CNBP), Adelaide, South Australia, 5000, Australia.
On-Demand Nanoliter Sampling Probe for the Collection of Brain Fluid.
Anal Chem
July 2022
Microsystems Laboratory 4 (STI-IEM-LMIS4), École Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland.
Continuous fluidic sampling systems allow collection of brain biomarkers . Here, we propose a new sequential and intermittent sampling paradigm using droplets, called Droplet on Demand (DoD). It is implemented in a microfabricated neural probe and alternates phases of analyte removal from the tissue and phases of equilibration of the concentration in the tissue.
View Article and Find Full Text PDFMass Activated Droplet Sorting (MADS) Enables High-Throughput Screening of Enzymatic Reactions at Nanoliter Scale.
Angew Chem Int Ed Engl
March 2020
Dept. of Chemistry, University of Michigan, 930 N University, Ann Abor, MI, 48109, USA.
Microfluidic droplet sorting enables the high-throughput screening and selection of water-in-oil microreactors at speeds and volumes unparalleled by traditional well-plate approaches. Most such systems sort using fluorescent reporters on modified substrates or reactions that are rarely industrially relevant. We describe a microfluidic system for high-throughput sorting of nanoliter droplets based on direct detection using electrospray ionization mass spectrometry (ESI-MS).
View Article and Find Full Text PDFStudy of aldehyde oxidase with phthalazine as substrate using both off-line and on-line capillary electrophoresis.
J Pharm Biomed Anal
February 2019
KU Leuven - University of Leuven, Department of Pharmaceutical and Pharmacological Sciences, Pharmaceutical Analysis, Herestraat 49, O&N2, PB 923, 3000, Leuven, Belgium. Electronic address:
An optimized and economical capillary electrophoretic method for both off-line and on-line study of the enzyme aldehyde oxidase and its substrate phthalazine was developed. The separation of the substrate phthalazine and its metabolite 1-phthalazinone was achieved using micellar electrokinetic chromatography (MEKC) with sodium dodecyl sulphate in the background electrolyte (BGE). The BGE consists of 25 mM sodium phosphate buffer containing 50 mM sodium dodecyl sulphate at pH 7.
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