The detoxifying effect of Polygonum equisetiforme extracts against dichlorvos (DDVP)-induced oxidative stress and neurotoxicity in the commercial clam Ruditapes decussatus.

Effects of Polygonum equisetiforme extracts against dichlorvos were investigated in the commercial clam Ruditapes decussatus. The toxicity of this pesticide was firstly tested in R. decussatus gill and digestive gland tissues using five doses varying from 0.05 to 1 mg/l during 2, 4, and 7 days. Results showed that 0.05 mg/l of DDVP induced oxidative stress and neurotoxicity in R. decussatus after 2 days of exposure. Investigations of the effects of P. equisetiforme extracts in R. decussatus exposed to 0.25 mg/l of DDVP were made in clams receiving three concentrations (0.009, 0.045, and 0.09 g/l) during 4 and 7 days. Antioxidant enzymes SOD and CAT as well as HO content and AChE were quantified by colorimetric method. Four days of exposure to DDVP increased SOD and CAT activities and enhances HO content. AChE levels decreased considerably following DDVP exposure, although a restoration in the enzyme activity was observed with P. equisetiforme extract (E3 = 0.09 g/l). Overall, P. equisetiforme extract at concentration (E1 = 0.009 g/l) prevents oxidative stress caused by DDVP, while 0.09 g/l of P. equisetiforme extract induced an effect similar to that obtained with DDVP alone. Nevertheless, this concentration was found effective for the restoration of the AChE activity.