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High-yield Production of Amyloid-β Peptide Enabled by a Customized Spider Silk Domain. | LitMetric

AI Article Synopsis

Article Abstract

During storage in the silk gland, the N-terminal domain (NT) of spider silk proteins (spidroins) keeps the aggregation-prone repetitive region in solution at extreme concentrations. We observe that NTs from different spidroins have co-evolved with their respective repeat region, and now use an NT that is distantly related to previously used NTs, for efficient recombinant production of the amyloid-β peptide (Aβ) implicated in Alzheimer's disease. A designed variant of NT from Nephila clavipes flagelliform spidroin, which in nature allows production and storage of β-hairpin repeat segments, gives exceptionally high yields of different human Aβ variants as a solubility tag. This tool enables efficient production of target peptides also in minimal medium and gives up to 10 times more isotope-labeled monomeric Aβ peptides per liter bacterial culture than previously reported.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6959368PMC
http://dx.doi.org/10.1038/s41598-019-57143-xDOI Listing

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