AI Article Synopsis
- The study investigates how Pt- and Pd-based anticancer drugs, specifically cisplatin and PdSpm, affect DNA at a molecular level.
- Researchers used various techniques like quasi-elastic neutron scattering (QENS), inelastic neutron scattering (INS), Fourier transform infrared (FTIR), and microRaman spectroscopy to analyze changes in DNA's structure and dynamics.
- Results showed that cisplatin mainly affects the spine of hydration around DNA, while PdSpm has a significant impact on the dynamics of the DNA backbone, highlighting the importance of hydration in understanding these drugs' mechanisms.
Article Abstract
The mode of action of Pt- and Pd-based anticancer agents (cisplatin and PdSpm) was studied by characterising their impact on DNA. Changes in conformation and mobility at the molecular level in hydrated DNA were analysed by quasi-elastic and inelastic neutron scattering techniques (QENS and INS), coupled to Fourier transform infrared (FTIR) and microRaman spectroscopies. Although INS, FTIR and Raman revealed drug-triggered changes in the phosphate groups and the double helix base pairing, QENS allowed access to the nanosecond motions of the biomolecule's backbone and confined hydration water within the minor groove. Distinct effects were observed for cisplatin and PdSpm, the former having a predominant effect on DNA´s spine of hydration, whereas the latter had a higher influence on the backbone dynamics. This is an innovative way of tackling a drug´s mode of action, mediated by the hydration waters within its pharmacological target (DNA).
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7024343 | PMC |
| http://dx.doi.org/10.3390/molecules25020246 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!